๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Effects of EGF and calcium on adult parenchymal hepatocyte proliferation

โœ Scribed by Peter M. Eckl; Wendi R. Whitcomb; George Michalopoulos; Randy L. Jirtle

Publisher
John Wiley and Sons
Year
1987
Tongue
English
Weight
460 KB
Volume
132
Category
Article
ISSN
0021-9541

No coin nor oath required. For personal study only.

โœฆ Synopsis

Adult rat hepatocytes were grown in serum-free medium containing 0.05-4 mM Ca++ and 40 ng/ml EGF. After 48 hours of cultivation the mitotic index and the percentage of second division metaphases were determined. The results demonstrated a maximum proliferation response to EGF at a Ca++ concentration of 0.4 mM. With lower and higher external Ca+ + concentrations the fraction of cells undergoing more than one cell division decreased. At lower Ca+ + concentrations this decrease appears to result from a reduced viability. In contrast, the low response to EGF at higher C a + + concentrations-especially in the physiological range-may reflect the influence of Ca+ + on the state of hepatocyte differentiation.

Liver regeneration is one of the most studied models hepatocyte cultures would be useful for studies on sister of growth because it can be induced by a partial hepatec-chromatid exchange induction, and eventually, for tomy and it can be compared to the growth of neoplastic achieving conditions that would allow clonal growth of liver. The use of culture systems in which hepatocytes hepatocytes in culture.

๐Ÿ“œ SIMILAR VOLUMES

Effects of rapamycin on cultured hepatoc
Effects of rapamycin on cultured hepatocyte proliferation and gene expression
โœ Antonio Francavilla; Brian I. Carr; Thomas E. Starzl; Alessandro Azzarone; Giuse ๐Ÿ“‚ Article ๐Ÿ“… 1992 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 718 KB

Rapamycin, a potent immunosuppressive drug that disrupts normal signal-transduction processes, inhibited hepatocyte proliferation without evidence of inherent cytotoxicity in rat hepatocytes cultured in conventional medium or in a medium enriched with epidermal growth factor. The antiproliferative e

Effects of hormones and serum on glycoge
Effects of hormones and serum on glycogen metabolism in adult rat liver parenchymal cell primary cultures
โœ P. Roy Walker; Marie J. Grindle ๐Ÿ“‚ Article ๐Ÿ“… 1977 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 701 KB

## Abstract Parenchymal cells from adult rat liver, isolated by a collagenase perfusion technique, have been maintained in primary culture and a detailed study on carbohydrate metabolism carried out over the initial 48โ€hour culture period. The glucose concentration of the medium exerts a major infl

The effect of extracellular calcium and
The effect of extracellular calcium and magnesium on the proliferation of murine lymphoblasts
โœ James K. Brennan; Marshall A. Lichtman ๐Ÿ“‚ Article ๐Ÿ“… 1973 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 814 KB

## Abstract We have shown that the murine lymphoblast, L5178Y, requires extracellular magnesium or calcium for proliferation in suspension culture. Although both cations produce biphasic effects on growth, magnesium is the more potent since it: (1) stimulates proliferation at lower concentrations;

Effects of dexamethasone on albumin and
Effects of dexamethasone on albumin and collagen gene expression in primary cultures of adult rat hepatocytes
โœ Douglas M. Jefferson; Dr. Lola M. Reid; Marie-Adele Giambrone; David A. Shafritz ๐Ÿ“‚ Article ๐Ÿ“… 1985 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 883 KB

To further our studies on collagen gene expression, we have evaluated the molecular basis for the finding that steroids decrease collagen synthesis in cultured hepatocytes. We studied the effects of dexamethasone on primary cultures of adult rat hepatocytes grown on tissue culture plastic in either