Effects of density-dependent inhibition of growth on phospholipid metabolism in monolayer cultures of animal cells
β Scribed by William R. Gallaher; Herbert A. Blough
- Book ID
- 115702974
- Publisher
- Elsevier Science
- Year
- 1976
- Tongue
- English
- Weight
- 672 KB
- Volume
- 173
- Category
- Article
- ISSN
- 0003-9861
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The effect of cell crowding on DNA synthesis (incorporation of 3HTdR and 32P04) was studied by an improved method in monolayers of secondary cells and established cell lines, either normal or transformed by viruses or carcinogens. The method was based mainly on pulse labeling of cultures of cells a
## Abstract Suspension cultures of Lβ929 fibroblasts grown to densities of 6 to 10 Γ 10^6^ cells/ml through daily centrifugation and resuspension in fresh media, have been maintained for periods up to five months without change in viability or cell size. DNA synthesis and mitosis in these cultures