Effects of cytochalasin H on chick embryo explants cultured in vitro
β Scribed by Surendra Ghaskadbi; Leela Mulherkar
- Publisher
- Elsevier Science
- Year
- 1984
- Tongue
- English
- Weight
- 608 KB
- Volume
- 33
- Category
- Article
- ISSN
- 0300-483X
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The effects of cytochalasin B (CB), a microfilament inhibitor, and demecolcine (DC), a microtubule inhibitor, on freeze-thaw survival and culture survival of early cleavage stage mouse embryos, was evaluated. In the first experiment, eight-cell mouse embryos were frozen in dimethylsulfoxide (DMSO) o
The effect of supplementary L-methionine (Met) on the incorporation of methionine was evaluated in 9.5-day rat conceptuses cultured in vitro. Parallel experiments with L-leucine (Leu) were performed for comparison. Conceptuses were cultured for 24 hr in the presence of 3 H-labeled Met or Leu, and th