Effects of colicin E2 on DNA and the bacterial membrane in vivo
โ Scribed by P.S. Ringrose
- Book ID
- 115745183
- Publisher
- Elsevier Science
- Year
- 1973
- Weight
- 621 KB
- Volume
- 312
- Category
- Article
- ISSN
- 0005-2787
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The DNA damage in intact Staphylococcus aureus and E. coli cells induced by photosensitized deuteroporphyrin or hemin is described. Treatment of S. aureus cultures with hemin or photosensitized deuteroporphyrin (Dp) caused time-dependent changes in the plasmidial DNA profiles. The major observation
Base substitutions have been introduced into the segment of the colicin E1 gene corresponding to the polypeptide region between the 404th and the 502nd residues which was considered to participate in colicin E1 export and bacteriocin activity. The methods used were in vitro localized mutagenesis wit