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Effects of coexisting neurochemicals on the release of serotonin from the intermediate area of rat thoracic spinal cord

✍ Scribed by Ling Yang; Cinda J. Helke

Publisher
John Wiley and Sons
Year
1995
Tongue
English
Weight
605 KB
Volume
21
Category
Article
ISSN
0887-4476

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✦ Synopsis

Serotonin (5-HT), substance P (SP), neurokinin A (NKA), and thyrotropin-releasing hormone (TRH) coexist in the nerve terminals of the intermediolateral cell column (IML) of the thoracic spinal cord. The Ca2+-dependent release of 5-HT from the microdissected intermediate area (including the IML) of the rat thoracic spinal cord, and the ~-H T ~B autoreceptor regulator of 5-HT release, were previously demonstrated. In this paper, the effects of SP, NKA, TRH, and/or their analogs on the release of ["]5-HT from the intermediate area were investigated using a n in vitro superfusion system. Both SP (the endogenous ligand for neurokinin-1 (NK,) receptor) and a n NK, agonist (GR 73632) significantly increased the basal release of L3H]5-HT. SP and GR 73632 did not change the K+-stimulated release of L3H15-HT. The effect of the NK, agonist on the basal release of [3H]5-HT was dose-dependent, was reduced by a n NK, antagonist (GR 823341, and was not dependent on extracellular Ca". Neither NKA, a n NK, agonist (GR 64349), nor a TRH analog (MK-771) altered the basal or stimulated release of [3H]5-HT. These data suggest that basal release of 5-HT from the intermediate area of the rat thoracic spinal cord is regulated by SP (acting through a n NK, receptor), but not by NKA or TRH. These results provide evidence for the role of SP as a modulator of serotoninergic neurons in the intermediate area of the thoracic spinal cord, and may help to clarify the role of coexisting neurochemicals in the spinal regulation of the sympathetic nervous system. 0 1995 Wiley-Liss, Inc.*

MATERIALS AND METHCIDS

Animal housing Adult male Sprague-Dawley rats (220-250 g) were obtained from Taconic Farms (Germantown, NY). The

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