Effects of Cd2+ and two cadmium organic complexes on isolated rat liver mitochondria
✍ Scribed by Sergei M. Korotkov; Vadim V. Glazunov; Eugenii V. Rozengart; Andrei A. Suvorov; Elena R. Nikitina
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 144 KB
- Volume
- 13
- Category
- Article
- ISSN
- 1095-6670
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✦ Synopsis
Effects of Cd 2 and two complexes of bivalent cadmium with 1,3-bis(4-chlorbenzylidenamino)-guanidine and anabasine on ion permeability of the inner membrane and respiration of isolated rat liver mitochondria were studied. Starting from 5 lM, Cd 2 decreased state 3 and DNP-stimulated respiration of mitochondria and increased their state 4 respiration. At 30 lM, Cd 2 decreased state 4 respiration. The complexes, particularly complex of Cd 2 with 1,3-bis(4chlorbenzylidenamino)-guanidine, inhibited the mitochondrial respiration at lower concentration of Cd 2 . Nonenergized mitochondria incubated in media containing 125 mM of NH 4 NO 3 or KNO 3 showed more pronounced swelling in experiments with 10 lM of the complexes than with Cd 2 . The complexes produced swelling of the mitochondria energized by 5 mM of succinate and incubated in medium containing 25 mM K-acetate and 100 mM sucrose. Uptake of 137-Cs by succinate-energized mitochondria in the presence of 10 8 M of valinomycin was substantially decreased in experiments with 10 lM of the complexes than with Cd 2 . Ruthenium red (7.5 lM) prevented this effect with 10 lM of complex of Cd 2 with 1,3-bis(4-chlorbenzylidenamino)-guanidine and especially complex of Cd 2 with anabasine and Cd 2 . These results indicate that the cadmium organic complexes affect respiration and perturb ion permeability significantly stronger than Cd 2 .