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Effects of calcium, alginate, and calcium-alginate immobilization on growth and tropane alkaloid levels of a stable suspension cell line ofDatura innoxiaMill

✍ Scribed by E. Gontier; B. S. Sangwan; J. N. Barbotin


Book ID
104675289
Publisher
Springer
Year
1994
Tongue
English
Weight
408 KB
Volume
13
Category
Article
ISSN
0721-7714

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✦ Synopsis


A stabilized two-year old suspension of a Datura innoxia cell line, producing small amounts of tropane alkaloids (scopolamine and hyoscyamine) was used in this study. Calcium alginate immobilization has been shown to be able to increase secondary metabolite (i. e. alkaloid) production. The effects of calcium and ungellified alginate were both beneficial for tropane alkaloid synthesis; a 10mM calcium chloride supply gave the best results, with a 10-fold yield increase.