Effects of Ca++ mobilization on expression of androgen-regulated genes: Interference with androgen receptor-mediated transactivation by AP-1 proteins
✍ Scribed by Murtha, Patricia E.; Zhu, Wen; Zhang, Jianye; Zhang, Shaobo; Young, Charles Y.F.
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 279 KB
- Volume
- 33
- Category
- Article
- ISSN
- 0270-4137
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✦ Synopsis
BACKGROUND.
The adult prostate is maintained through an equilibrium between cell growth and death rates. Androgen deprivation induces an increase in intracellular Ca ++ , AP-1 gene expression of androgen-inducible genes. METHODS. Northern blot analysis, band-shift assays, and transient cotransfection assays were used to study the effects of Ca ++ mobilizer A23187 on gene expression in human prostate cancer cells. RESULTS. A23187 repressed androgen-upregulated mRNAs for prostate-specific antigen (PSA) and hKLK2, and rapidly induced mRNA levels for c-fos and c-jun. AP-1 protein-DNA binding activities were elevated after A23187 treatments. Androgen receptor (AR)-mediated induction of chloramphenicol acetyltransferase (CAT) reporter was repressed by AP-1 proteins. CONCLUSIONS. The repression of AR-mediated induction of PSA and hKLK2 genes by Ca ++ mobilizers is due to the interference of AR transactivation activity by AP-1 proteins.