Effects of bone protein extract on human mesenchymal stem cells proliferation and differentiation

✍ Scribed by Charlotte Woo; Haisheng Li; Anette Baatrup; Andrea Krause; Moustapha Kassem; Cody Bunger; Martin Lind

• Publisher: John Wiley and Sons
• Year: 2006
• Tongue: English
• Weight: 136 KB
• Volume: 79A
• Category: Article
• ISSN: 1549-3296
• DOI: 10.1002/jbm.a.30892

✦ Synopsis

Abstract

Since its osteoinductive capacity has been established, demineralized bone matrix is considered a suitable alternative to bone autograft in the healing of osseous defects. The mechanisms of bone formation induction are still not fully understood. In this study we assessed the effects of a dispersion of bovine bone extract COLLOSS® (BPE) with regard to proliferation and differentiation of a human mesenchymal stem cell line overexpressing human telomerase reverse transcriptase (hMSC‐TERT). Proliferation rate was determined by ^3^H‐thymidine incorporation. The differentiation of hMSC‐TERT cells to osteoblastic cells was assessed by means of measuring alkaline phosphatase activity and collagen synthesis in vitro. Both undifferentiated and osteoblast‐differentiated hMSC‐TERT cells were investigated for response to BPE. The metabolic responses to BPE were compared to unstimulated cells and cells stimulated with bovine collagen (COL). Undifferentiated hMSC‐TERT cells responded to BPE with increased proliferation and decreased alkaline phosphatase activity. Osteoblastic differentiated hMSC‐TERT cells had a diminished proliferative response and an increased alkaline phosphatase activity and collagen synthesis. Our study demonstrated significant metabolic effects of BPE on hMSC‐TERT cells, which were highly dependant on the differentiated state of the cells. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2006