Effects of autolysis on properties of μ- and m-calpain
✍ Scribed by Hongqi Li; Valery F. Thompson; Darrel E. Goll
- Book ID
- 113485602
- Publisher
- Elsevier Science
- Year
- 2004
- Tongue
- English
- Weight
- 880 KB
- Volume
- 1691
- Category
- Article
- ISSN
- 0167-4889
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The presence of the calpain-calpastatin system in human umbilical vein endothelial cells (HUVEC) was investigated by means of ion exchange chromatography, Western blot analysis, and Northern blot analysis. On DEAE anion exchange chromatography, calpain and calpastatin activities were eluted at appro
Proteolytic digestion by trypsin and chymotrypsin was used to probe conformation and domain structure of the mu- and m-calpain molecules in the presence and the absence of Ca(2+). Both calpains have a compact structure in the absence of Ca(2+); incubation with either protease for 120 min results in