Effects of autoclave-induced carbohydrate hydrolysis on the growth ofBeta vulgariscells in suspension

✍ Scribed by Heidi Sawyer; Ke-Cheng Hsiao

• Publisher: Springer
• Year: 1992
• Tongue: English
• Weight: 388 KB
• Volume: 31
• Category: Article
• ISSN: 0167-6857
• DOI: 10.1007/bf00043479

✦ Synopsis

Media of de

Greef & Jacobs (1979)

were autoclaved either with all the nutrient components in a single vessel (medium 1) or with the following components in separate vessels: FeNa-EDTA + CaCI 2 (medium 2), FeNa-EDTA+NaHEPO 4 (medium 3) or sucrose (medium 4). Medium 5 was prepared by autoclaving FeNa-EDTA + NaH2PO 4 and sucrose in two separate vessels. It was found that the dry mass yield of cell suspensions of Beta vulgaris was lowest in medium 1, followed by media 2 and 3.

There was no significant difference among media 3, 4, and 5.

The plot of dry mass yield of the cell suspensions against the rates of cyanide-initiated oxygen consumption which indicate the extent of carbohydrate hydrolysis of the media during autoclaving, indicated the presence of a threshold rate of about 17-20nmolml -I min -1. Dry mass yield of the suspensions decreased rapidly when the rate exceeded this value.

For media with glucose as the source of carbohydrate, the rate of cyanide-initiated oxygen consumption exceeded the threshold value by a factor of 1.5 to 2, depending on the volume of the media autoclaved.