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Effects of ascorbic acid, alpha-tocopherol, and glutathione on microspore embryogenesis inBrassica napusL.

✍ Scribed by Hoseini, Maryam; Ghadimzadeh, Mortaza; Ahmadi, Behzad; Teixeira da Silva, Jaime A.

Book ID
121535157
Publisher
Society for In Vitro Biology
Year
2013
Tongue
English
Weight
374 KB
Volume
50
Category
Article
ISSN
1475-2689

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Effects of culture density, conditioned
Effects of culture density, conditioned medium and feeder cultures on microspore embryogenesis inBrassica napusL. cv. Topas
✍ Bin Huang; Sharon Bird; Roger Kemble; Daina Simmonds; Wilf Keller; Brian Miki πŸ“‚ Article πŸ“… 1990 πŸ› Springer 🌐 English βš– 509 KB

In microspore cultures of Brassica napus L. cv. Topas, embryo yield increases with culture density up to about 40,000 microspores per ml. A much higher density (100,000 per ml) appears inhibitory to embryogenesis. A relatively high culture density (30,000 or 40,000 per ml) for the first 2-4 days of