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Effects of adenosine 3′5′-cyclic monophosphoric acid on the morphology, growth and cell cycle of earle's L cells

✍ Scribed by Joyce Taylor-Papadimitriou

Publisher
John Wiley and Sons
Year
1974
Tongue
French
Weight
578 KB
Volume
13
Category
Article
ISSN
0020-7136

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✦ Synopsis

Abstract

The morphology of Earle's L cells is radically altered after incubation with adenosine 3′5′‐cyclic monophosphate (cyclic AMP) and cell growth is inhibited. The concentration of nucleotide required to completely inhibit cell growth depends on the initial cell density although the breakdown of cyclic AMP in the supernatant fluid to adenosine is independent of the cell density. A decrease in mitotic index is noted several hours after the addition of cyclic AMP, while a reduction in the fraction of cells in S is only observed after 2 days. Upon removal of the drug an increase in the number of cells synthetizing DNA (as detected autoradiographically) is observed well before the increase of mitotic rate. These results suggest that cells are blocked in moving through S to G2. Cells resume normal growth rates and morphology only 6–8 days after the removal of cyclic AMP, but can then be propagated indefinitely.

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