Effects of abscisic acid (ABA) on the polymerase activity have been investigated. The specific activity of the I~NA polymerase enzyme decreased when coleoptiles were preincubated for 6 hours or longer in 3.8 X 10 -5 M ABA. Inhibition of RNA synthesis could however already be detected after 3 hours A
Effects of abscisic acid on nucleic acid metabolism in maize coleoptiles
โ Scribed by J. H. M. Bex
- Publisher
- Springer-Verlag
- Year
- 1972
- Tongue
- English
- Weight
- 595 KB
- Volume
- 103
- Category
- Article
- ISSN
- 0032-0935
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โฆ Synopsis
Following treatment with ABA an inhibition of total I~NA synthesis was observed after 30 hours. Total soluble ribonuclease activity did not change during the first 8 hours, after which an increase could be observed.
Separation of nucleic acids with polyacrylamide gel electrophoresis indicated that synthesis of soluble RNA was less inhibited by ABA than synthesis of ribosomal RNA.
Effects of 5-FU and ABA on ribosomal RNA precursor were investigated. It could be shown that 5-FU did not inhibit ribosomal precursor synthesis, but that ABA did so.
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Plastic and elastic in-vitro extensibilities (Epl and Eol) of cell walls from growing maize (Zea mays L.) coleoptile segments were measured by stretching frozen-thawed tissue, pre-extended to its in-vivo length, at constant force (creep test) in a custom-buildt extensiometer, equipped with a linear-
Plastic and elastic in-vivo extensibilities (gpl and Eel, respectively) of cell walls of growing maize (Zea mays L.) coleoptile segments were measured by stretching living tissue at constant force (creep test) in an extensiometer. The linear displacement transducer used as a measuring device permits
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