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Effects of aberrations and specimen structure in conventional, confocal and two-photon fluorescence microscopy

โœ Scribed by R. D. SIMMONDS; T. WILSON; M. J. BOOTH


Book ID
108866950
Publisher
John Wiley and Sons
Year
2011
Tongue
English
Weight
378 KB
Volume
245
Category
Article
ISSN
0022-2720

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We investigated the effect of a finite-sized confocal pinhole on the performance of nonlinear optical microscopes based on two-photon excited fluorescence and second-harmonic generation. These techniques were implemented using a modified inverted commercial confocal microscope coupled to a femtoseco