Effects of a single injection of anti-asialo GM1 serum on natural cytotoxicity and the growth of a regressive colonic tumor in syngeneic rats
✍ Scribed by T. Shimizu; H. Pelletier; A. Hammann; N. O. Olsson; M. S. Martin; F. Martin
- Publisher
- John Wiley and Sons
- Year
- 1987
- Tongue
- French
- Weight
- 614 KB
- Volume
- 40
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
The REGb tumor cell line is a cloned variant of the D H D -K I 2 cell line, established from a colon carcinoma chemically induced in the rat. Unlike the parent DHD-KIZ cell line, or other clones, which give progressive tumors when inoculated to the syngeneic rat, REGb cells produce tumors which regress in 3 to 5 weeks and never cause metastasis. In order to explore the role of natural killer (NK) cells in REGb tumor regression, each rat was given one injection of anti-asialoGM I (anti-asGMI) serum, a known inhibitor of N K activity. This injection was done 24 hr before REGb cell challenge. This injection significantly depressed the in vitro cytotoxicity of peripheral blood lymphocytes on REGb cells for 2 weeks. REGb tumors grew larger and regressed later in the treated animals than those in the controls. Furthermore, a progressive or recurrent tumor was observed in 4 out of 10 treated rats, giving lung and/or lymph-node metastases in 2 cases.
Immuno-histological study of the cells infiltrating the REGb tumors in control and treated animals showed a decrease number of asGMl + and OX8+ lymphocytes, presumably N K cells, after anti-asGM I treatment. An increase in number of macrophages was demonstrated in the progressive tumors of treated animals. These results suggest that N K cells play an important role in the initial stage of the regression TSb tumors in untreated syngeneic rats.
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