Effects of a preformed extracellular matrix on long-term serum-free bone marrow culture
โ Scribed by L. Teofili; M. Sargiacomo; M. S. Iovino; G. Zini; G. Leone; B. Bizzi; C. Peschle
- Publisher
- Springer
- Year
- 1992
- Tongue
- English
- Weight
- 425 KB
- Volume
- 65
- Category
- Article
- ISSN
- 0939-5555
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The stromal cell layer is believed to play an important role in long-term human bone marrow cultures (LTHBMCs). At present, neither the role that the stromal cell extra-cellular matrix (ECM) plays in influencing stroma behavior is well understood nor are the effects of stroma aging. Rapid medium exc
## Abstract The roles of colonyโstimulating factors in longโterm bone marrow cultures were studied and compared. After single additions of high concentrations of unpurified colonyโstimulating activities to the cultures, rapid deterioration of the cultures was observed. This appears to result from c