Effects of 2,4-dinitrophenol and anoxia on the inorganic-pyrophosphate content of the spadix ofArum maculatumand the root apices ofPisum sativum
✍ Scribed by Jane E. Dancer; Tom ap Rees
- Publisher
- Springer-Verlag
- Year
- 1989
- Tongue
- English
- Weight
- 442 KB
- Volume
- 178
- Category
- Article
- ISSN
- 0032-0935
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✦ Synopsis
This work was done to determine whether the inorganic-pyrophosphate (PPi) content of plant tissues changes when the rate of glycolysis is altered. Treatment of excised clubs of the spadix of Arum maeulatum L. and root apices of Pisum sativum L. with 2,4-dinitrophenol increased the rates of respiration but had no detectable effects on PPi contents. When the two tissues were subjected to up to 60 min anoxia, no changes in PPi were detected. Anoxia was shown to lead to a fall in ATP and concomitant rises in ADP and AMP in pea roots. It is argued (i) that variation in the rate of glycolysis was not accompanied by detectable changes in PPi content, (ii) that this observation does not favour the view that pyrophosphate fructose 6-phosphate 1-phosphotransferase mediates appreciable entry into glycolysis, and (iii) that PPi content can be maintained when respiratory-chain phosphorylation is inhibited.