Effector and target cells in the assessment of natural cytotoxic activity of rhesus monkeys

✍ Scribed by Gabriele R. Lubach; Christopher L. Coe; Joseph W. Karaszewski; William B. Ershler

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 990 KB
• Volume: 39
• Category: Article
• ISSN: 0275-2565
• DOI: 10.1002/(sici)1098-2345(1996)39:4<275::aid-ajp7>3.0.co;2-w

✦ Synopsis

In humans, decreased natural killer cell (NK) activity has been associated with stressful life events, whereas acute arousal and disturbance frequently has been reported to result in increased NK activity. This bidirectional immune modulation prompted us to investigate the effects of a social stressor on the lymphocyte cytolytic activity of 31 infant rhesus monkeys. The first of three studies evaluated the effects of an 8 hr maternal separation on the infants' cytolytic response against the K562 target-cell line. A finding of increased lytic activity indicated a need for a longer evaluation-after a 24 hr separation-and an additional assessment of two other target-cell lines, Raji and Daudi. The observation of decreased lytic responses to Raji and Daudi, in association with increased lysis of K562, warranted a third study to delineate which rhesus effector cells were responsible for lysis of the K562 and Raji target cells. By isolating cell subsets, it was possible to observe that the majority of unprimed cytotoxic activity resided in the CD3-population of cells, but that the CD3 + CD8 + population also mediated a significant amount of cytotoxicity against both targets. In conclusion, these findings support earlier studies indicating that maternal separation results in significant immune alterations in infant monkeys. However, the complex nature of changes in cytotoxic responses during prolonged stress revealed that different lymphocyte populations engage in parallel and compensatory alterations.