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Effecters on human placental alkaline phosphatase activity

✍ Scribed by M. G. Roig; N. I. Ghaïs; F. J. Burguillo; J. M. Cachaza; J. F. Kennedy

Publisher
John Wiley and Sons
Year
1993
Tongue
English
Weight
678 KB
Volume
31
Category
Article
ISSN
0959-8103

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✦ Synopsis

Abstract

A kinetic study has been carried out on the effects of enzyme concentration, protein (human serum albumin) concentration and ionic strength (inorganic and organic sodium salts) on the activity of human placental alkaline phosphatase in solution. The activity is depressed by serum albumin, and the corresponding complex of the protein with the substrate, decreasing its concentration available for catalysis, is responsible for an effect similar to competitive inhibition of the alkaline phosphatase activity. Activating and inhibiting effects were found for inorganic anions, respectively.

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