Effect on platelet-derived growth factor-induced mitogenesis of double-stranded RNA: Evidence for an autocrine growth inhibition mediated by interferon-β
✍ Scribed by Karin Forsberg; Ylva Paulsson; Bengt Westermark
- Publisher
- John Wiley and Sons
- Year
- 1988
- Tongue
- English
- Weight
- 728 KB
- Volume
- 136
- Category
- Article
- ISSN
- 0021-9541
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✦ Synopsis
Stimulation of normal human foreskin fibroblasts with platelet-derived growth factor (PDGF) was inhibited by the addition of the synthetic double-stranded RNA polyinosinic-polycytidylic acid (poly-l:C) as measured by incorporation of 3Hthymidine (3H-TdR). Single-stranded polycytidylic or polyinosinic acid had no effect. Double-stranded RNA is an inducer of interferon+ (IFN-p) in fibroblasts.
On the mRNA level, an expression of IFN-P2 but not of IFN-pl was seen after addition of PDCF and/or poly-l:C. The inhibition of PDGF-induced mitogenesis was completely blocked by an antiserum to IFN-P. Poly-l:C did not interfere with PDGF binding to its receptor, nor did it block protein synthesis, indicating that the inhibition is not due to a nonspecific toxic effect of the double-stranded RNA but rather is mediated by IFN-P. The present study implies that the IFN-P system in fibroblasts is a very potent autocrine inhibitory pathway.