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Effect ofl-azetidine 2-carboxilic acid on the activity of the general amino-acid permease fromSaccharomyces cerevisiaevar.ellipsoideus

✍ Scribed by Rosario Iglesias; Jose Miguel Ferreras; Francisco Javier Arias; Raquel Muñoz; Ma Angeles Rojo; Tomás Girbés

Publisher
Springer
Year
1991
Tongue
English
Weight
464 KB
Volume
155
Category
Article
ISSN
0302-8933

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✦ Synopsis

Addition of the L-proline analogue L-azetidine 2-carboxylic acid to growing cultures of Saccharomyces cerevisiae var. ellipsoideus promoted fast deactivation of the general aminoacid permease, measured as L-valine uptake, without an immediate decrease in the growth rate. Cells preincubated with the analogue for 3 h were unable to restore either growth ability or general aminoacid permease activity in analogue-free medium. Eadie-Hofstee plots of L-valine uptake in the presence of the analogue are consistent with a strong reduction in the number of active molecules of the general amino-acid permease located in the plasma membrane. Inhibitory effects on protein synthesis were seen after preincubations of the yeast with the analogue for 3 h although a 30 min preincubation had no effect.

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