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Effect of γ-irradiation on soya bean proteins

✍ Scribed by Myung-Woo Byun; Il-Jun Kang; Yukako Hayashi; Yasuki Matsumura; Tomohiko Mori

Publisher
John Wiley and Sons
Year
1994
Tongue
English
Weight
544 KB
Volume
66
Category
Article
ISSN
0022-5142

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✦ Synopsis

Abstract

The properties of soya bean proteins following γ‐irradiation (5–20 kGy) were investigated in connection with protein denaturation. Irradiation doses above 10 kGy caused a decrease in 7S and 11S components and an increase in 2S and 15S components (P < 0.05). However, subunit patterns determined by electrophoresis were not changed appreciably over the entire irradiation dose range. In the differential scanning calorimetry thermogram, the denaturation temperatures of 11S and 7S components were not affected by γ‐irradiation, while increased irradiation dose caused a decrease in the enthalpy values of 11S and 7S components due to protein denaturation. Changes in the circular dichroism spectra and tryptophan fluorescence intensity (P < 0.05) were observed only at 20 kGy.

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