Effect of water activity on enzymatic synthesis of cephalexin
β Scribed by Chang K. Hyun; Jung H. Kim; Young J. Kim
- Book ID
- 105045952
- Publisher
- Springer Netherlands
- Year
- 1989
- Tongue
- English
- Weight
- 203 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0141-5492
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
In an enzymatic synthesis of cephalexin (CEX) using an acylase from Xanthomonas citri, the effect of polyethylene glycol (PEG) on the synthetic reaction of Zamino-3-deacetoxycephalosporanic acid (FADCA) and D-alpha-phenylglycine methyl ester (PGM) to CEX was investigated. The addition of PEG (MW 300
Cephalexin was synthesized in aqueous two-phase systems (ATPSs) by using penicillin G acylase (PGA) (penicillin amidohydrolase, EC3.5.1.11) as a catalyst and 7-aminodeacetoxicephalosporanic acid (7-ADCA) and phenylglycine methyl ester (PGME) as substrates. In the ATPS composed of 20% (w/w) PEG 400 a
## Abstract A cascade of two enzymatic transformations is employed in a oneβpot synthesis of cephalexin. The nitrile hydratase (from __R. rhodochrous__ MAWE)βcatalyzed hydration of Dβphenylglycine nitrile to the corresponding amide was combined with the penicillin G acylase (penicillin amidohydrola