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Effect of VK framework-1 glycosylation on the binding affinity of lymphoma-specific murine and chimeric LL2 antibodies and its potential use as a novel conjugation site

โœ Scribed by Shui-On Leung; Arnold S. Dion; Matthew C. Pellegrini; Michele J. Losman; Ruth C. Grebenau; David M. Goldenberg; Hans J. Hansen


Publisher
John Wiley and Sons
Year
1995
Tongue
French
Weight
884 KB
Volume
60
Category
Article
ISSN
0020-7136

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Effect of VK framework-1 glycosylation o
โœ Shui-On Leung; Arnold S. Dion; Matthew C. Pellegrini; Michele J. Losman; Ruth C. ๐Ÿ“‚ Article ๐Ÿ“… 1995 ๐Ÿ› John Wiley and Sons ๐ŸŒ French โš– 884 KB

A potential asparagine (Am)-linked glycosylation site was identified in the VK FRI sequence of an anti-B lymphoma monoclonal antibody (MAb), L U . SDS-PAGE analysis and endo-F treatment of both murine and chimeric LL2 antibodies indicated that this site was glycosylated; however, no differences in t