Primary cultures of guinea pig ear keratinocytes were treated with vitamin A acid at plating, or at 14 h or 14 days after plating. The intracellular content of the cyclic nucleotides cAMP and cGMP was determined by radioimmunoassay at intervals during a period of 50 h after treatment. When added at
Effect of vitamin A acid on the growth of keratinocytes in culture
β Scribed by David I. Wilkinson
- Publisher
- Springer-Verlag
- Year
- 1978
- Tongue
- English
- Weight
- 373 KB
- Volume
- 263
- Category
- Article
- ISSN
- 0340-3696
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β¦ Synopsis
The effect of vitamin A acid on the culture of guinea pig ear keratinocytes was investigated. Use of this agent and treatment of the plastic dishes with collagen solution facilitated attachment and growth. Vitamin A acid elicited a wave of DNA synthesis which was studied by incorporation of tritiated thymidine and quantitation of cellular DNA, particularly during the period 24--45 h after plating. During this period, thymidine incorporation into DNA was increased ten-fold by vitamin A acid and showed a complex pattern of peaks and shoulders which may represent the S phase of synchronous keratinocytes.
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