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Effect of vitamin A acid on the growth of keratinocytes in culture

✍ Scribed by David I. Wilkinson


Publisher
Springer-Verlag
Year
1978
Tongue
English
Weight
373 KB
Volume
263
Category
Article
ISSN
0340-3696

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✦ Synopsis


The effect of vitamin A acid on the culture of guinea pig ear keratinocytes was investigated. Use of this agent and treatment of the plastic dishes with collagen solution facilitated attachment and growth. Vitamin A acid elicited a wave of DNA synthesis which was studied by incorporation of tritiated thymidine and quantitation of cellular DNA, particularly during the period 24--45 h after plating. During this period, thymidine incorporation into DNA was increased ten-fold by vitamin A acid and showed a complex pattern of peaks and shoulders which may represent the S phase of synchronous keratinocytes.


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