Effect of vacuum drying on protein-mannitol interactions: The physical state of mannitol and protein structure in the dried state
β Scribed by Vikas K. Sharma; Devendra S. Kalonia
- Book ID
- 111767129
- Publisher
- Springer US
- Year
- 2004
- Tongue
- English
- Weight
- 610 KB
- Volume
- 5
- Category
- Article
- ISSN
- 1530-9932
- DOI
- 10.1208/pt050110
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Isoperibol calorimetry was used to evaluate protein/carbohydrate interactions after freeze drying. rh-DNase, rh-GH, rh-MetGH, and rh-IGF-I were freeze dried with either mannitol, sucrose, trehalose, or dextran at concentrations ranging from 0% to 100%(w/w). Enthalpies of solution for both freeze-dri
The effects of various sugars on the structural stabilization of protein during freeze-drying were investigated. The degree of native structure of protein that was freeze-dried and rehumidified at constant relative humidities (RHs) was evaluated by measurement of the alpha-helix content by Fourier-t