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Effect of tunicamycin on N-acetyl-β-d-glucosaminidase produced by Trichoderma harzianum

✍ Scribed by C.J. Ulhoa; D. Sankievicz; P.S. Limeira; J.F. Peberdy


Book ID
117480838
Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
159 KB
Volume
1528
Category
Article
ISSN
0304-4165

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Endo-N-acetyl-P-D-glucosaminidase H (Endo H) was purified to homogeneity (3000-fold) from a culture filtrate of Streptomyces plicatus. The key step was substrate-affinity chromatography, which afforded a lOOO-fold purification and yielded a protease-and exoglycosidase-free preparation of Endo H. Pro