Effect of tumor necrosis factor α on electrically induced calcium transients elicited in C2C12 skeletal myotubes

✍ Scribed by Lisa N. van Kann; Anthony J. Bakker

• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 83 KB
• Volume: 35
• Category: Article
• ISSN: 0148-639X
• DOI: 10.1002/mus.20635

✦ Synopsis

Abstract

Diseases involving chronic inflammation can lead to prolonged exposure of skeletal muscle to inflammatory cytokines such as tumor necrosis factor α (TNFα), which may contribute to the skeletal muscle weakness seen in these conditions. In this study we examined the effect of a prolonged exposure to TNFα on intracellular Ca^2+^ transients elicited in skeletal C~2~C~12~ myotubes. A 48‐h exposure to TNFα (10 ng/mL) significantly reduced the peaks, time to peak, and rate of Ca^2+^ decay of electrically induced Ca^2+^ transients elicited in C~2~C~12~ skeletal myotubes. TNFα exposure had no significant effect on the resting Ca^2+^ levels. The results of this study indicate that prolonged exposure to TNFα decreases sarcoplasmic reticulum Ca^2+^ release in cultured skeletal muscle cells. This altered Ca^2+^ release could contribute to the muscle weakness found in conditions involving chronic inflammation. Muscle Nerve, 2006