A number of techniques are currently employed for the fractionation of heterogeneous cell populations or for the separation of cells in different phases of their cycle. With the development of osmotically inert colloidal silica particles media, density gradient centrifugation became an established m
Effect of tulipin on cell cycle progression analyzed by BrdUrd incorporation
β Scribed by M. Vitale; S. Papa; L. Roncuzzi; A. Gasperi-Campani
- Publisher
- Springer
- Year
- 1990
- Tongue
- English
- Weight
- 258 KB
- Volume
- 93
- Category
- Article
- ISSN
- 1432-119X
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β¦ Synopsis
The effect of tulipin, a protein from plant origin recently purified, on cell cycle progression has been analyzed in the sensitive EUE cells by BrdUrd incorporation. The cytofluorimetric results demonstrate that tulipin specifically interacts with the S phase, with a dose-dependent decrease of the total S phase cells and an increase of the G1/G2 cells after 4 h of treatment in the synchronized EUE cells, whereas in the asynchronous population it mainly causes a dose-dependent decrease in the incorporation of BrdUrd per cell.
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