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Effect of tulipin on cell cycle progression analyzed by BrdUrd incorporation

✍ Scribed by M. Vitale; S. Papa; L. Roncuzzi; A. Gasperi-Campani


Publisher
Springer
Year
1990
Tongue
English
Weight
258 KB
Volume
93
Category
Article
ISSN
1432-119X

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✦ Synopsis


The effect of tulipin, a protein from plant origin recently purified, on cell cycle progression has been analyzed in the sensitive EUE cells by BrdUrd incorporation. The cytofluorimetric results demonstrate that tulipin specifically interacts with the S phase, with a dose-dependent decrease of the total S phase cells and an increase of the G1/G2 cells after 4 h of treatment in the synchronized EUE cells, whereas in the asynchronous population it mainly causes a dose-dependent decrease in the incorporation of BrdUrd per cell.


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