𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Effect of trypsin and chymotrypsin on polypeptides of human rotavirus KUN strain

✍ Scribed by T. Sato; S. Kitaoka; H. Suzuki; T. Konno; N. Ishida

Publisher
Springer-Verlag
Year
1987
Tongue
English
Weight
512 KB
Volume
176
Category
Article
ISSN
0300-8584

No coin nor oath required. For personal study only.

✦ Synopsis

In view of the fact that trypsin enhances the infectivity of human rotavirus and decreases its hemagglutination, the trypsin-mediated structural modification of the viral polypeptides was analysed, using the KUN strain, a cultivable human rotavirus isolate, grown in the absence of trypsin. A major polypeptide sensitive to trypsin treatment was Vp4 with a molecular weight of 80,000, being cleaved into three polypeptides with molecular weights of 54,000 (P54), 30,000 (P30), and 24,000 (P24). Vp4 was also sensitive to chymotrypsin treatment, generating cleavage products different from those obtained with trypsin.

πŸ“œ SIMILAR VOLUMES

Effect of strain on human keratinocytes
Effect of strain on human keratinocytes in vitro
✍ Teiji Takei; Carlos Rivas-Gotz; Chrys A. Delling; Jason T. Koo; Ira Mills; Thoma πŸ“‚ Article πŸ“… 1997 πŸ› John Wiley and Sons 🌐 English βš– 317 KB πŸ‘ 2 views

Tissue expansion, a technique to enlarge the skin surface area with an expandable balloon, has been widely used in reconstructive surgery. Although the effect of tissue expansion on in vivo skin physiology and histology has been well documented, it remains unclear whether keratinocytes or other cell