Effect of trimethylamine on acetate utilization byMethanosarcina barkeri

✍ Scribed by Michael Blaut; Gerhard Gottschalk

• Book ID: 104765038
• Publisher: Springer
• Year: 1982
• Tongue: English
• Weight: 644 KB
• Volume: 133
• Category: Article
• ISSN: 0302-8933
• DOI: 10.1007/bf00415007

✦ Synopsis

During growth of Methanosarcina barkeri strain Fusaro on a mixture of trimethylamine and acetate, methane production and acetate consumption were biphasic. In the first phase trimethylamine (33 mmol • 1-1) was depleted and some acetate (11-14 from 50 mmol x 1 1) was metabolized simultaneously. In the second phase the remaining acetate was cleaved stoichiometrically into CH4 and CO2. Kinetic experiments with (2-14C)acetate revealed that only 2.5 % of the methane produced in the first phase originated from acetate : 18 % of the acetate metabolized was cleaved into CH 4 and C02, 23 % of the acetate was oxidized, and 55 % was assimilated. Methane produced from CD3-COOH in the first phase consisted of CD2H2 and CD3H in a ratio of 1 : 1.