Effect of thyroid hormone administration on the depletion of circulating glutathione in the isolated perfused rat liver and its relationship to basolateral γ-glutamyltransferase activity
✍ Scribed by Videla, Luis A. ;Fernández, Virginia
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 768 KB
- Volume
- 10
- Category
- Article
- ISSN
- 0887-2082
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✦ Synopsis
The influence of thyroid hormone administration on liver glutathione (GSH) extraction in the isolated perfused liver was studied in fed rats for a period of 1-7 days following a single dose of 0.1 mg 3,5,3'-triiodothyronine (T3)/kg. T3 treatment led to an early and transient calorigenic response, as well as an enhancement in liver GSH removal, reaching a maximal effect at 2 days after hormone administration, which was normalized in the 3-to 7-day period studied. Addition of the yglutamyltransferase (y-GT) inhibitor DL-serineborate (4 mM) to the perfusate abolished the increase in the hepatic removal of GSH elicited by T3, and enhanced the sinusoidal concentration of GSH, studied at 2 days after hormone administration. These data support the role of hepatic basolateral y-GT ectoactivity in the depletion of portally added and liver-derived GSH as an adaptive response to recover GSH levels after reduction by T,-induced oxidative stress.