Effect of the methoxyiminoacetamide fungicide, SSF129, on respiratory activity in Botrytis cinerea

✍ Scribed by Tamura, Hiroto; Mizutani, Akira; Yukioka, Hideo; Miki, Nobuo; Ohba, Katsuaki; Masuko, Michio

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 115 KB
• Volume: 55
• Category: Article
• ISSN: 1526-498X
• DOI: 10.1002/(sici)1096-9063(199907)55:7<681::aid-ps16>3.0.co;2-8

✦ Synopsis

(2,5-dimethylphenoxymethyl)

phenyl]acetamide (SSF129) has been developed as a broad-spectrum systemic fungicide for control of cereal and fruit diseases. This compound inhibited NADH-oxidation by submitochondrial particles from mycelial cells of Botrytis cinerea, with an EC 50 value of 14.5 nM, due to blockage of electron transport through the cytochrome bc 1 complex in the mitochondrial respiratory chain. However, SSF129 did not suppress, but rather increased, oxygen consumption by mycelial cells of the fungus. This was because mycelial cells contain an alternative oxidase protein and the cells have the ability to rapidly switch electron ¯ux from the main cytochrome pathway to the alternative pathway on blockage of the former by SSF129. The alternative pathway of the mycelia seems not to be operative when the cytochrome pathway is functional. Naturally occurring ¯avonoids inhibited the alternative oxidase of the mycelial cells in a dose-dependent manner, with EC 50 values of 68.4 mM for ¯avone and 63.7 mM for ¯avanone. These observations suggested that plant components play an important role in control of gray mould by SSF129.