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Effect of the different supramolecular organization on the uptake and metabolization of exogenous GM1 ganglioside by human fibroblasts

✍ Scribed by Antonella Giglioni; Vanna Chigorno; Marina Pitto; Manuela Valsecchi; Paola Palestini; Riccardo Ghidoni

Publisher: Elsevier Science
Year: 1990
Tongue: English
Weight: 413 KB
Volume: 55
Category: Article
ISSN: 0009-3084
DOI: 10.1016/0009-3084(90)90080-b

No coin nor oath required. For personal study only.

✦ Synopsis

In this report we have investigated the differences in the uptake and metabolization of exogenous GMI by human fibroblasts, as a function of its supramolecular organization in solution. For this we used a tritium labelled GMI, given alone or inserted in dispersions of phosphatidylcholine (PC) or sulphatide. The addition of fetal calf serum (FCS) to these dispersions was also studied. With respect to GM1 pure micelles, the presence in the medium of a sulphatide/GMl, 10 : 1 molar ratio, greatly increased the incorporation of GMl-associated radioactivity by the cultured cells. Conversely, the presence of PC dramatically diminished the GMI incorporation values. The metabolization of exogenous GMI was favoured by the presence of FCS, regardless of the presence of sulphatide. The obtained data provide useful information on the appropriate procedure for feeding cultured fibroblasts with gangliosides.

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