In the chromatographic separation of a-cyclodextrin and glucose, the parameters relating to the adsorption equilibria and rate processes of the solutes were evaluated for the sodium forms of cation-exchange resins with contents of divinylbenzene (DVB) of 2,4,6 and 8%. By using these parameters, the chromatograms of the solutes were then calculated. It is concluded that the resin with 6% DVB is most suitable for the chromatographic separation of the solutes on a preparative scale.
INTRODUCIION
Cyclodextrins (CDs) are cyclic and non-reducing maltooligosaccharides produced from starch by cyclodextrin glucosyltransferases. They have an ability to form complexes with guest compounds, and have been utilized in food and pharmaceutical industries. The enzymes, regardless of their origins, produce a mixture of a-, /3-and y-CDs and maltooligosaccharides'. In order to produce a specific CD, a separation process is indispensable.
/l-CD can easily be separated from other saccharides due to its low solubility. The co-operative action of taka-amylase and glucoamylase hydrolyzes y-CD and maltooligosaccharides to glucose, but the enzymes do not affect c&D'. Therefore, production of a-CD with high purity requires the separation of a-CD and glucose.
Among separation procedures, chromatography may be promising because of the non-use of additives. Lammers3 demonstrated the separation by adsorption