Effect of the anticodon loop size of yeast alanyl tRNA on its biological activity

✍ Scribed by Youxin Jin; Musui Qiu; Wenqin Li; Keqin Zeng; Junru Bao; Peijuan Gong; Renlong Wu; Debao Wang (T.P. Wang)

• Publisher: Elsevier Science
• Year: 1987
• Tongue: English
• Weight: 731 KB
• Volume: 161
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(87)90474-x

✦ Synopsis

Three analogs of yeast alanyl tRNA with anticodon loops of different sizes, tRNA75 (no G35 and S-terminal phosphate), tRNA77 (one more C between G35 and C36, no 5'-terminal phosphate), and ptRNA79 (with Cm'I$ between G35 and C36), were synthesized. In comparison with the reconstituted natural yeast tRNA, the charging activities of the three analogs were 90% (tRNA75), 94.7% (tRNA77), and 104% (ptRNA79). These results supported the conclusion (Yang De-ping and Wang De-bao (T. P. Wang) (1983) Acta Biochim. Biophys. Sin. 15, 83-90) that the anticodon loop of yeast alanyl tRNA was not involved in the interaction between alanyl-tRNA synthetase from rat liver and yeast alanyl tRNA. In contrast, in the rabbit reticulocyte lysate system, the incorporation of alanine in the charged analogs was 0% (tRNA75 and ptRNA79) and 100% (tRNA77). There were significant differences between the incorporation activities of analogs and those of the reconstituted molecule. The reason for these differences is discussed. 0 1987 Academic Press. II-K.