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Effect of tetranitromethane on the aldolase and β-decarboxylase activities of bovine liver 2-keto-4-hydroxyglutarate aldolase

✍ Scribed by Roger S. Lane; Eugene E. Dekker


Book ID
118851057
Publisher
Elsevier Science
Year
1969
Tongue
English
Weight
377 KB
Volume
36
Category
Article
ISSN
0006-291X

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Detection of aldolase activity on polyac
✍ Neil D. Lewinski; Eugene E. Dekker 📂 Article 📅 1978 🏛 Elsevier Science 🌐 English ⚖ 935 KB

A method is described for the detection of 2-keto-4-hydroxyglutarate aldolase activity after electrophoresis of the enzyme on polyacrylamide gels. When gels are incubated with substrate (2-keto-4-hydroxyglutarate), activity is seen as a yellow-colored band due to interaction' of the product (glyoxyl