Effect of temperature variation on sister chromatid exchange frequency in cultured human lymphocytes
β Scribed by T. K. Pandita
- Publisher
- Springer
- Year
- 1983
- Tongue
- English
- Weight
- 166 KB
- Volume
- 63
- Category
- Article
- ISSN
- 0340-6717
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## Abstract The utility of the sister chromatid exchange (SCE) assay for human population studies is potentially limited by the variability associated with individual baseline SCE frequencies. This investigation identifies and quantifies the major sources of preparative and biological variation ass
The incidence of sister chromatid exchange (SCE) was determined in human lymphocytes cultured with fetal calf, human AB, and autologous serum. In each individual studied, cells grown in medium supplemented with fetal calf and human AB serum showed higher yields of SCE than those cultured with autolo
Lymphocytes from 20 notmal subjects (11 male and 9 female) were examined for the frequency and location of sister chromatid exchanges (SCE) by the BrdU--Giemsa method. The mean frequency of SCE was 6.37 with little significant variation. One subject had a high number of exchanges in chromosome 1 whi