Effect of temperature on separation efficiency for high-speed size exclusion chromatography
β Scribed by Renn, Curtiss N.; Synovec, Robert E.
- Book ID
- 126322221
- Publisher
- American Chemical Society
- Year
- 1992
- Tongue
- English
- Weight
- 780 KB
- Volume
- 64
- Category
- Article
- ISSN
- 0003-2700
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
On the basis of studying the retention and band broadening of proteins on the TSK SW column, diffusion coefficients (OS) of solute in stationary phase were obtained which elucidate the hydrodynamic process of chromatographic resolution of proteins by hydrophilic size-exclusion chromatography (SEC).
The size-dependent separation of viral and subviral particles in the range lo'-IO7 daltons was undertaken by high-performance liquid chromatography. A combination of Ultrahydrogel 2000 and 1000 size-exclusion columns, equilibrated and developed with Tris buffer (pH 7.4) was used to fractionate extr