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Effect of stage of development on survival of mouse embryos frozen-thawed rapidly

✍ Scribed by A. Massip; P. Van Der Zwalmen; F. Leroy


Book ID
115850199
Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
327 KB
Volume
21
Category
Article
ISSN
0011-2240

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Survival of mouse embryos frozen-thawed
✍ Miyamoto, Hajime ;Ishibashi, Takehiko πŸ“‚ Article πŸ“… 1983 πŸ› John Wiley and Sons 🌐 English βš– 422 KB πŸ‘ 1 views

Eight-cell mouse embryos were frozen by using glycerol, ethylene glycol, or erythritol as the cryoprotectant. The samples were slowly cooled (0.5"C/min) to temperatures between -10 and -79Β°C before direct transfer to liquid nitrogen (-196Β°C) and thawed slowly ( -20"C/min) or rapidly ( -500Β°C/min). T