Membrane proteins of human erythrocytes can be phosphorylated not only by membrane casein kinase (MS) but also by cytosolic casein kinases CS and CTS, resembling casein kinase I and II, respectively. Casein kinase CS, like membrane casein kinase MS, preferentially phosphorylates membrane proteins su
โฆ LIBER โฆ
Effect of spermine on membrane-associated and membrane-inserted forms of protein kinase C
โ Scribed by Maria Stella Moruzzi; Gaetano Marverti; Giorgio Piccinini; Chiara Frassineti; Maria Giuseppina Monti
- Publisher
- Springer
- Year
- 1993
- Tongue
- English
- Weight
- 749 KB
- Volume
- 124
- Category
- Article
- ISSN
- 0300-8177
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This paper describes a simple and direct procedure for assaying Ca(2+)-dependent protein kinase C (PKC) activity in membrane fractions isolated from purified murine B lymphocytes (B cells) treated with phorbol 12-myristate 13-acetate (PMA). The results indicate that membrane-bound PKC in B cells, tr