Effect of sperm number and oxygenation state of the storage media on in vitro fertility of bovine sperm stored at ambient temperature
β Scribed by J. Krzyzosiak; P. Molan; L. McGowan; R. Vishwanath
- Book ID
- 117270167
- Publisher
- Elsevier Science
- Year
- 2001
- Tongue
- English
- Weight
- 771 KB
- Volume
- 55
- Category
- Article
- ISSN
- 0093-691X
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The effects of storage of hamster sperm in vitro a t temperatures above freezing on fertilization and early development were studied. Initially, female hamsters woere 5seminated y-tificially with epididymal sperm stored up t o five days at 5 , 16 , or 23-25 C. Eggs were examined at the pronuclear st
## Abstract Rat epididymal spermatozoa, at a concentration of 40β65 Γ 10^4^ cells/ml, were preincubated for 5β5.5 hr and then added to eggs from superovuβlated, immature rats. By 10β12 hr after insemination, 86% of the eggs had undergone fertilization, but many of them (41%) were polyspermic. When