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Effect of sonication on paraffin-embedded tissue preparation for DNA flow cytometry

✍ Scribed by Nick J. Gonchoroff; John J. Ryan; Teresa K. Kimlinger; Thomas E. Witzig; Philip R. Greipp; John S. Meyer; Jerry A. Katzmann

Publisher
John Wiley and Sons
Year
1990
Tongue
English
Weight
477 KB
Volume
11
Category
Article
ISSN
0196-4763

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Background: Flow cytometry of single-cell suspensions prepared by enzymatic digestion from formalin-fixed, paraffin-embedded tissue suffers from several major drawbacks. The most important factors that influence the results are the high and unpredictable coe Β¨fficients of variation (CVs) of the G 0