Effect of sodium fluoride and sodium pyruvate on palatal developmentin vitro

✍ Scribed by Myers, Gordon S.

• Publisher: John Wiley and Sons
• Year: 1971
• Tongue: English
• Weight: 921 KB
• Volume: 171
• Category: Article
• ISSN: 0003-276X
• DOI: 10.1002/ar.1091710104

✦ Synopsis

Explants of the embryonic rat palate have been treated by adding sodium fluoride and sodium pyruvate to the culture medium. Fluoride, at specific concentrations, causes retardation of palatal shelf growth so that fusion does not occur during the culture period. Partial or complete fusion does occur if fluoride levels are reduced.

Sodium pyruvate added to the medium advances the time of fusion of explants over that found in controls. When combined with fluoride in the medium, pyruvate can reverse the effects of fluoride on shelf growth and permit complete fusion to take place in a large percentage of explants.

The mode of action of fluoride or pyruvate under these experimental conditions has not been determined. However, the known effects of fluoride as an enzyme inhibitor must be considered.