𝔖 Bobbio Scriptorium
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Effect of silver nitrate on long-term culture and regeneration of callus fromBrassica oleraceavar.gemmifera

✍ Scribed by J. Williams; D. A. C. Pink; N. L. Biddington

Publisher
Springer
Year
1990
Tongue
English
Weight
528 KB
Volume
21
Category
Article
ISSN
0167-6857

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✦ Synopsis

Incorporation of AgNO 3 (1-10mg 1 -l) into the culture medium of Brassica oleracea var. gemmifera callus significantly improved growth and allowed long-term callus culture. In the absence of AgNO3, callus died shortly after removal from the hypocotyl explants. Regeneration of shoots from callus on low-hormone medium was also enhanced by AgNO 3 . Significant differences in shoot production were found between the three genotypes examined. Cv. Aries produced large numbers of shoots even in the absence of AgNO3. Investigation of callus production from the inbred parent lines of cv. Aries indicated that 'tissue culturability' may be determined genetically.

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