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Effect of sesamin on growth and arachidonic acid content of neoplastic and non-neoplastic prostate epithelial cell cultures

✍ Scribed by Gareth Griffiths; Helen E. Jones; Colby L. Eaton; Sakayu Shimizu; A. Keith Stobart

Publisher: John Wiley and Sons
Year: 1998
Tongue: English
Weight: 151 KB
Volume: 12
Category: Article
ISSN: 0951-418X
DOI: 10.1002/(sici)1099-1573(199809)12:6<417::aid-ptr334>3.0.co;2-9

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✦ Synopsis

Sesamin (a lignan present in sesame oil) is reported to specifically inhibit D5 desaturation and the formation of arachidonic acid (20:4n-6) from dihomogamma-linolenic acid (20:3n-6) in cell free extracts. In this study the effects of sesamin have been evaluated on cell cultures derived from canine prostatic tissues of epithelial origin from neoplastic and non-neoplastic tissues. Sesamin reduced cell proliferation in a dose dependent manner in both cell lines with TD 50 values of 91 AE 16 mM (non-neoplastic) and 132 AE 12 mM (neoplastic). In the non-neoplastic cells, supplemented with gamma-linolenic acid (18:3n-6) in the culture media, sesamin had little effect on the content of C16 and C18 fatty acids but markedly reduced the content of 20:4n-6 and caused an accumulation of 20:3n-6. This observation is consistent with sesamin inhibiting D5 desaturase activity. Sesamin, however, had little effect on the content and ratio of 20:3n-6 and 20:4n-6 fatty acids in neoplastic cells indicating that D5 desaturase activity was low in these cells. Thus, sesamin is an effective compound for reducing D5 desaturase activity in non-neoplastic cell cultures and could be used to manipulate the content of series 1 and 2 prostaglandins derived from 20:3n-6 and 20:4n-6, respectively.

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