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Effect of serum concentration, type of culture medium and pH on the lysosomal enzyme activity of cultured human amniotic fluid cells

โœ Scribed by J. Butterworth; G.R. Sutherland; D.M. Broadhead; A.D. Bain


Book ID
115820257
Publisher
Elsevier Science
Year
1974
Tongue
English
Weight
539 KB
Volume
53
Category
Article
ISSN
0009-8981

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## Abstract The tyrosinase (EC 1.14.18.1) activity of cultured mouse melanoma cells B16 in the stationary phase of growth, depends greatly on the pH of the medium and the kind of sugar present. The enzyme activity of a homogenate of cells grown at pH 7.2 in Eagles's MEM supplemented with 10% new b