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Effect of serum concentration on production of monoclonal antibodies and on shear sensitivity of a hybridoma

✍ Scribed by L. Pol; G. Zijlstra; M. Thalen; J. Tramper

Publisher
Springer
Year
1990
Tongue
English
Weight
422 KB
Volume
5
Category
Article
ISSN
1615-7605

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✦ Synopsis

The effect of serum content of culture medium on the specific production rate of monoclonal antibodies (Mab's) and on shear sensitivity has been studied with hybridoma's, cultured in a continuous stirred tank reactor (CSTR). No decrease in specific Mab-production was found when the serum concentration was reduced from 10 to 2.5%, while steady state cell concentrations were hardly affected as well. In contrast the cell death rate in a bubble column strongly increased when the serum concentration was lowered, which could be ascribed to a reduced physical protective effect by the serum.

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