Effect of salicin on induction and carbon catabolite repression of endoxylanase synthesis inPenicillium janthinellumMTCC 10889
✍ Scribed by Kundu, Aditi; Ray, Rina Rani
- Book ID
- 125406627
- Publisher
- Versita
- Year
- 2014
- Tongue
- English
- Weight
- 199 KB
- Volume
- 68
- Category
- Article
- ISSN
- 0366-6352
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
Amongst various carbon sources, xylan was found to be the sole inducer of endoxylanase production by Penicillium janthinellum MTCC 10889 in submerged cultivation. Endoxylanase synthesis by a xylan induced culture was initially repressed after a simultaneous addition of xylose, probably by the inducer exclusion mechanism, but it was resumed and achieved its highest level at a much later stage of growth (at 120 h). Xylose added after 30 h of growth cannot exert its full repressive effect. Although glucose was proved to be a more potent repressor than xylose, supplementation of salicin, an alcoholic β-glycoside containing d-glucose, with pure xylan resulted in an about 3.22 fold increase in the enzyme synthesis at 72 h followed by constant high production of the enzyme at least until the 144th h of growth. Inducing capacity of salicin in a xylan induced culture was significantly reduced when it was added after 30 h of growth. Addition of salicin and xylan help to partially overcome the repressive effect of xylose and glucose. Failure of salicin in recovering the endoxylanase synthesis in actinomycin D and cyclohexamide inhibited the xylan induced culture indicating that salicin cannot initiate the de novo synthesis of the enzyme.